The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence

Profilin-1, a member of the Profilin family, is ubiquitously expressed protein that controls actin polymerization in concentration-dependent manner. As mutations Profilin-1 gene have potential implications neurodegenerative disease progression, well-characterized anti-Profilin-1 antibodies would be beneficial to scientific community. In this study, we characterized sixteen commercial for Western blot, immunoprecipitation, and immunofluorescence applications, using standardized experimental protocol based on comparing read-outs knockout cell lines isogenic parental controls. We identified many high-performing encourage readers use report as guide select most appropriate antibody their specific needs.